Abstract
Level of selected antibacterial tear proteins in children with diabetes type 1
- Klinika Okulistyki Dziecięcej Katedry Pediatrii Zabiegowej Uniwersytetu Medycznego w Łodzi Samodzielny Publiczny Zespół Opieki Zdrowotnej Uniwersyteckiego Szpitala Klinicznego Nr 4 Uniwersytetu Medycznego w Łodzi
- Klinika Pediatrii, Onkologii, Hematologii i Diabetologii I Katedry Pediatrii Uniwersytetu Medycznego w Łodzi Samodzielny Publiczny Zespół Opieki Zdrowotnej Uniwersyteckiego Szpitala Klinicznego Nr 4 Uniwersytetu Medycznego w Łodzi
Introduction
Antibacterial immunity in diabetes is impaired, which increases the risk of general and local infections. The aim of the study was to evaluate non-specific local antibacterial immunity based on lactoferrin and lysozyme concentration in tears in children with diabetes type 1.
Material and methods
Children at the age of 10-18 years old were studied. Group 1. consisted of children without diabetes, group 2. included patients with new onset of diabetes and group 3. consisted of children with decade-long diabetes. Among all patients tears were collected from inferior coniunctival fornix with hematocrit glass capillaries in purpose to measure lactoferrin and lysozyme concentration. ELISA method was used in laboratory testing.
Results
Level of lactoferrin did not differ significantly among all groups. Concentration of lysozyme was statistically lower in group with decade-long diabetes (group 3.) compared to patients without diabetes. Mild correlation between lactoferrin and lysozyme levels was seen in individual patients in whole group of probands together.
Conclusions
Diabetes type 1 in children is associated with significant changes in concentration of tear proteins, which contribute to antibacterial immunity.
Keywords
Diabetes type 1, tears, antibacterial proteins, lactoferrin, lysozyme, ELISA test
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