Abstract
1/2012
vol. 114
Review paper
Application of atomic force microscopy (AFM) in ophthalmology
- Oddział Okulistyczny Okręgowego Szpitala Kolejowego w Katowicach
- Instytut Fizyki Doświadczalnej Uniwersytetu Wrocławskiego
- Zakład Fizyki Ciała Stałego Instytutu Fizyki Uniwersytetu Śląskiego w Katowicach
- Zakład Biofizyki i Fizyki Molekularnej Instytutu Fizyki Uniwersytetu Śląskiego w Katowicach
- Ophthalmology Unit, Department of Surgical Specialities, University of Messina, Italy
Klinika Oczna 2012, 114 (1)
Online publish date: 2012/03/29
Atomic force microscopy (AFM) allows to examine surface of different biological objects in the nearly physiological conditions at
the nanoscale. The purpose of this work is to present the history of introduction and the potential applications of the AFM in ophthalmology research and clinical practice. In 1986 Binnig built the AFM as a next generation of the scanning tunnelling microscope
(STM). The functional principle of AFM is based on the measurement of the forces between atoms on the sample surface and the
probe. As a result, the three-dimensional image of the surface with the resolution on the order of nanometres can be obtained.
Yamamoto used as the first the AFM on a wide scale in ophthalmology. The first investigations used the AFM method to study
structure of collagen fibres of the cornea and of the sclera. Our research involves the analysis of artificial intraocular lenses (IOLs).
According to earlier investigations, e.g. Lombardo et al., the AFM was used to study only native IOLs. Contrary to the earlier investigations, we focused our measurements on lenses explanted from human eyes. The surface of such lenses is exposed to the
influence of the intraocular aqueous environment, and to the related impacts of biochemical processes.
We hereby present the preliminary results of our work in the form of AFM images depicting IOL surface at the nanoscale. The
images allowed us to observe early stages of the dye deposit formation as well as local calcinosis. We believe that AFM is
a very promising tool for studying the structure of IOL surface and that further observations will make it possible to explain the
pathomechanism of artificial intraocular lens opacity formation.
the nanoscale. The purpose of this work is to present the history of introduction and the potential applications of the AFM in ophthalmology research and clinical practice. In 1986 Binnig built the AFM as a next generation of the scanning tunnelling microscope
(STM). The functional principle of AFM is based on the measurement of the forces between atoms on the sample surface and the
probe. As a result, the three-dimensional image of the surface with the resolution on the order of nanometres can be obtained.
Yamamoto used as the first the AFM on a wide scale in ophthalmology. The first investigations used the AFM method to study
structure of collagen fibres of the cornea and of the sclera. Our research involves the analysis of artificial intraocular lenses (IOLs).
According to earlier investigations, e.g. Lombardo et al., the AFM was used to study only native IOLs. Contrary to the earlier investigations, we focused our measurements on lenses explanted from human eyes. The surface of such lenses is exposed to the
influence of the intraocular aqueous environment, and to the related impacts of biochemical processes.
We hereby present the preliminary results of our work in the form of AFM images depicting IOL surface at the nanoscale. The
images allowed us to observe early stages of the dye deposit formation as well as local calcinosis. We believe that AFM is
a very promising tool for studying the structure of IOL surface and that further observations will make it possible to explain the
pathomechanism of artificial intraocular lens opacity formation.
Keywords
Atomic Force Microscopy, intraocular lens (IOL), nanotechnology
Integrated with
