The ef­fect of opso­nins on ery­th­ro­cy­tes pha­go­cy­to­sis in the vi­tre­ous-l­ab­or­at­ory re­se­arch (part A)

Purpose
We performed experimental investigations in vitro into the opsonization of Rh-positive erythrocytes by IgG anti-RhD antibodies and phagocytosis of erythrocytes in the vitreous body.

Material and methods
The vitreous has been taken from the corpses of young people. Anti-RhD antibodies came from preparations 'Gamma anti-D 150'. 1% Erytrocyte suspension of ccDee phenotype has been applied. Phagocytosis assay was performed in the medium of the vitreous and in Hank's solution.

Results
Phagocytosis assay performed in the vitreous and in Hank's solution did not display statistically significant differences.

Conclusions
The investigations in vitro presented here, proved that immunological reactions are really occurring in the vitreous body. They provide a base for the explanation of activity mechanism of anti-RhD antibodies injected intraoculary in cases of hemorrhages into vitreous body.